Table 1

MLST Primers and PCR Primers used in the amplification of the genes and the expected product sizes used in this study.

Gene PCR Primers

Product size

Gene Sequencing Primers


thrA: F 5'-GTCACGGTGATCGATCCGGT-3'

852 bp

thrA: sF 5'-ATCCCGGCCGATCACATGAT-3'

thrA: R 5'-CACGATATTGATATTAGCCCG-3'

thrA: sR 5'-CTCCAGCAGCCCCTCTTTCAG-3'

thrA: R1 5'-GTGCGCATACCGTCGCCGAC-3' (also Seq)

purE: F 5'-ATGTCTTCCCGCAATAATCC-3'

510 bp

purE: sF 5'-CGCATTATTCCGGCGCGTGT-3'

purE: R 5'-TCATAGCGTCCCCCGCGGATC-3'

purE: sF1 5'-CGCAATAATCCGGCGCGTGT-3'

purE: R1 5'-CGAGAACGCAAACTTGCTTC-3'

purE: sR 5'-CGCGGATCGGGATTTTCCAG-3'

purE: sR1 5'-GAACGCAAACTTGCTTCAT-3'

sucA: F 5'-AGCACCGAAGAGAAACGCTG-3'

643 bp

sucA: sF 5'-AGCACCGAAGAGAAACGCTG-3'

sucA: R 5'-GGTTGTTGATAACGATACGTAC-3'

sucA: sR 5'-GGTTGTTGATAACGATACGTAC-3'

hisD: F 5'-GAAACGTTCCATTCCGCGCAGAC-3'

894 bp

hisD: sF 5'-GTCGGTCTGTATATTCCCGG-3'

hisD: R 5'-CTGAACGGTCATCCGTTTCTG-3'

hisD: sR 5'-GGTAATCGCATCCACCAAATC-3'

aroC: F 5'-CCTGGCACCTCGCGCTATAC-3'

826 bp

aroC: sF 5'-GGCACCAGTATTGGCCTGCT-3'

aroC: R 5'-CCACACACGGATCGTGGCG-3'

aroC: sR 5'-CATATGCGCCACAATGTGTTG-3'

hemD: F 5'-ATGAGTATTCTGATCACCCG-3'

666 bp

hemD: sF 5'-GTGGCCTGGAGTTTTCCACT-3'

hemD: F1 5'-GAAGCGTTAGTGAGCCGTCTGCG-3'

hemD: sF1 5'-ATTCTGATCACCCGCCCCTC-3'

hemD: R 5'-ATCAGCGACCTTAATATCTTGCCA-3'

hemD: sR 5'-GACCAATAGCCGACAGCGTAG-3'

dnaN: F 5'-ATGAAATTTACCGTTGAACGTGA-3'

833 bp

dnaN: sF 5'-CCGATTCTCGGTAACCTGCT-3'

dnaN: R 5'-AATTTCTCATTCGAGAGGATTGC-3'

dnaN: sR 5'-CCATCCACCAGCTTCGAGGT-3'

dnaN: R1 5'-CCGCGGAATTTCTCATTCGAG-3' (also Seq)


Stepan et al. BMC Microbiology 2011 11:153   doi:10.1186/1471-2180-11-153

Open Data