Table 1

Strains and plasmids used in this study

Strain

Relevant characteristic

Source


Rhizobium

CFN42

wild type R. etli (pRet42a to pRet42f)

[58]

CFN2001

CFN42 lacking pRet42a and pRet42d

[37]

CFNX195

CFN42 derivative cured of pRet42a, pRet42d::Tn5mob

[32]

GR64

wild type bean-nodulating S. fredii (pSfr64a, pSfr64b)

[12]

GR64-1

GR64/pSfr64a::Tn5-GDYN, pSfr64b::Tn5mob

This work

GR64-2

GR64 cured of pSfr64a, pSfr64b::Tn5mob

This work

GR64-3

GR64-2 with pRet42a::Tn5-GDYN

This work

GR64-4

GR64 cured of pSfr64a and pSfr64b, RifR

This work

GR64-5

GR64-4/pRet42a::Tn5-GDYN

This work

GR64-6

GR64-4/pSfr64a::Tn5-GDYN

This work

CFN2001-1

CFN2001/pSfr64b::Tn5mob

This work

CFN2001-2

CFN2001/pSfr64b::Tn5mob, pRet42a::Tn5-GDYN

This work

CFN2001-3

CFN2001/pSfr64b::Tn5mob, pSfr64a::Tn5-GDYN

This work

Escherichia coli

DH5a

Receptor for transformation

[59]

S17-1

C600::RP-4-2 (Tc::Mu)(Km::Tn7)

[60]

S17/pDR21

Source of Tn5-GDYN

[17]

Agrobacterium tumefaciens

GMI9023

C-58 cured of its native plasmids

[35]

UIA143

recA pTi- derivative of C58

[61]

GMI9023/pSfr64a

GMI9023 with pSfr64a::Tn5-GDYN

This work

GMI9023/pSfr64b

GMI9023 with pSfr64b::Tn5mob

This work

Plasmids

pSUP5011

Tn5mob

[60]

pRK2013

Conjugation helper

[36]


Cervantes et al. BMC Microbiology 2011 11:149   doi:10.1186/1471-2180-11-149

Open Data