Additional file 4.
Fig. S4. Detection of Aap expression. Aap in lysostaphin-treated bacterial cells of SE1457ΔsaeRS, SE1457, and SE1457saec was detected by Western blot using an anti-Aap monoclonal antibody (made in our laboratory). Proteins were separated on 7% SDS-PAGE gels and then transferred to polyvinylidene fluoride (PVDF) membranes by electroblotting. Bands corresponding to Aap were quantified using the Quantity-one software. WT, SE1457; SAE, SE1457ΔsaeRS; SAEC, SE1457sae.
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Lou et al. BMC Microbiology 2011 11:146 doi:10.1186/1471-2180-11-146