Additional file 2.

Fig. S2. PIA detection in S. epidermidis biofilms. S. epidermidis strains were grown in 6-well plates under static conditions at 37°C for 24 h. Next, the cells were removed by scraping and collected by centrifugation before being resuspended in 0.5 M EDTA (pH 8.0). After proteinase K treatment (20 mg/mL) for 3 h at 37°C, serial dilutions of the PIA extracts were spotted onto PVDF membranes. Spots corresponding to PIA were quantified using the Quantity-one software. WT, SE1457; SAE, SE1457ΔsaeRS; SAEC, SE1457saec; 35984, S. epidermidis ATCC35984.

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Lou et al. BMC Microbiology 2011 11:146   doi:10.1186/1471-2180-11-146