Table 1

Proteins identified by HPLC-MS/MS

Band #

Sample

NCBI Accession

Name

Function


1

BCM

gi15924466

30S ribosomal protein S1 [Staphylococcus aureus subsp. aureus Mu50]

translation

1

BCM

gi227557405

elongation factor G [Staphylococcus aureus subsp. aureus MN8]

translation

2

BCM

gi15923949

glycerophosphoryl diester hosphodiesterase [Staphylococcus aureus subsp. aureus Mu50]

glycerophospholipid metabolism

3

BCM

gi15924653

valyl-tRNA synthetase [Staphylococcus aureus subsp. aureus Mu50]

translation

4

BCM

gi258423763

isoleucyl-tRNA synthetase Staphylococcus aureus A9635]

translation

5

BCM

gi2506027

N-acetyl-glucosaminidase [Staphylococcus aureus]

exoglycosidase

6

BCM

gi15924060

amidophosphoribosyltransferase Staphylococcus aureus subsp. aureus Mu50]

purine nucleotide biosynthesis

7

BCM

gi128852

Staphylococcal nuclease

nuclease

8

BCM

No significant hits

NA

NA

9

BCM

gi258424814

catalase [Staphylococcus aureus A9635]

antioxidant/oxidative stress

9

BCM

gi21282950

catalase [Staphylococcus aureus subsp. aureus MW2]

antioxidant/oxidative stress

10

BCM

No significant hits

NA

NA

11

BCM

No significant hits

NA

NA

12

BCM&PCM

gi15925406

phosphoglycerate mutase [Staphylococcus aureus subsp. aureus Mu50]

glycolysis

12

BCM&PCM

gi282917765

2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [Staphylococcus aureus subsp. aureus D139]

glycolysis

12

BCM&PCM

gi|15927092

6-phosphogluconate dehydrogenase [Staphylococcus aureus subsp. aureus N315]

Pentose phosphate

bifunctional 3-deoxy-7-hosphoheptulonate

12

BCM&PCM

gi15924727

synthase/chorismate mutase [Staphylococcus aureus subsp.

shikimate pathway

aureus Mu50]

12

BCM&PCM

gi15923310

glycerol ester hydrolase [Staphylococcus aureus subsp. aureus Mu50]

lipase

13

BCM&PCM

gi15924543

superoxide dismutase [Staphylococcus aureus subsp. aureus Mu50]

antioxidant/oxidative stress

14

BCM&PCM

gi15923346

5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [Staphylococcus aureus subsp. aureus Mu50]

methionine metabolism

14

BCM&PCM

gi293501167

aconitate hydratase 1 [Staphylococcus aureus subsp. aureus 58-424]

TCA

15

PCM

gi15925596

fructose-1,6-bisphosphate aldolase [Staphylococcus aureus subsp. aureus Mu50]

glycolysis

16

PCM

gi15923621

lipoprotein [Staphylococcus aureus subsp. aureus Mu50]

cell wall component

16

PCM

gi15925115

fructose-bisphosphate aldolase [Staphylococcus aureus subsp. aureus Mu50]

glycolysis

17

PCM

gi289550260

fructose-bisphosphate aldolase class II [Staphylococcus lugdunensis HKU09-01]

glycolysis

17

PCM

gi283470068

phosphoglycerate kinase [Staphylococcus aureus subsp. aureus ST398]

glycolysis

18

PCM

gi15923952

glucose-6-phosphate isomerase [Staphylococcus aureus subsp. aureus Mu50]

glycolysis

18

PCM

gi15923762

glyceraldehyde-3-phosphate dehydrogenase [Staphylococcus aureus subsp. aureus Mu50]

glycolysis

18

PCM

gi151221290

ornithine carbamoyltransferase [Staphylococcus aureus subsp. aureus str. Newman]

urea cycle


Proteins identified by HPLC-MS/MS analysis. Band numbers represent excised bands from 1D-SDS PAGE analysis of BCM and PCM (Figure 1).

Secor et al. BMC Microbiology 2011 11:143   doi:10.1186/1471-2180-11-143

Open Data