Influence of temperature on the antimicrobial activity of HydH5. A) HydH5 (20 μg) activity was tested at room temperature, 4°C, 37°C and 45°C by the standard CFU reduction analysis; B) HydH5 (20 μg) sensitivity to heat treatments (72°C,15 s; 72°C, 5 min; 100°C, 1 min; 100°C, 5 min). After the different treatments, the CFU reduction analysis was performed by challenging S. aureus Sa9 cells to the treated HydH5 at 37°C for 30 min. Error bars are the means ± standard deviations of three independent assays.
Rodríguez et al. BMC Microbiology 2011 11:138 doi:10.1186/1471-2180-11-138