Table 3

A summary of the binding of S. aureus polypeptides to immobilized ligands

Polypeptide tested

ELISA* cell-free supernatant

ELISA* purified 6xHis polypeptide

SPRpurified 6xHis polypeptide


ΔNarG

-

-

-

ΔFnBPA

Fn

-

Fn

ΔEbh

Fn

Fn

(Fn), Fg

ΔCoa

(Fn), Fg

(Fn), Fg,

Fg

ΔPurK

Fn, Fg

Fn, (CI)

Fn, Fg

ΔSCOR

(Fn), (Fg)

ND

ND

ΔUsp

Fn, Fg, (CIV)

(Fn)

Fg

ΔIspD

(Fn), (Fg)

ND

ND

ΔPBP

(Fn), (Fg)

-

-

D1-D3

Fn

ND

ND


* Binding of polypeptides to immobilized target molecules by enzyme-linked immunoassay

† Binding of polypeptides to immobilized target molecules by surface plasmon resonance

‡ C-terminally FLAG-tagged recombinant polypeptide used as positive control in screening of the FTP library

- indicate no binding; ligand in brackets indicate weak binding

ND, indicate not determined. The SCOR and IspD polypeptides could not be produced as 6xHis recombinant polypeptides and the D1-D3 polypeptide was produced into the cell-free growth medium and did not carry a His tag.

Kylväjä et al. BMC Microbiology 2011 11:117   doi:10.1186/1471-2180-11-117

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