Table 1

List of strains used for the validation of specificity of Campylobacter coli and Campylobacter jejuni real-time PCR assays

Bacterial species (n)

Name or origin of strain

C. coli real-time PCR identification

C. jejuni real-time PCR identification


Campylobacter coli (2)

CCUG 11283, CIP 7081

Positive

Negative

C. coli pig isolates (25)

Anses, ENVN-INRA

Positive

Negative

C. coli poultry isolates (25)

Anses, ENVN-INRA

Positive

Negative

C. coli human isolates (25)

Anses, CNR-CH

Positive

Negative

Campylobacter jejuni subsp jejuni (3)

CCUG 11284, NCTC 11168, NCTC 81176

Negative

Positive

C. jejuni

CIP 103726

Negative

Positive

C. jejuni poultry isolates (25)

Anses, ENVN-INRA

Negative

Positive

C. jejuni human isolates (25)

Anses, ENVN-INRA, CNR-CH

Negative

Positive

Campylobacter fetus subsp fetus (2)

CCUG 68231, CIP 2595396

Negative

Negative

Campylobacter fetus subsp venerealis

CCUG 33899

Negative

Negative

Campylobacter hyointestinalis

CCUG 14169

Negative

Negative

Campylobacter lari (3)

CCUG 23947, ATCC 35222, CIP 107080

Negative

Negative

Campylobacter upsaliensis (2)

CCUG 14913, CIP 400

Negative

Negative

Campylobacter sputorum

CIP 103749

Negative

Negative

Helicobacter canis

CIP 104753

Negative

Negative

Helicobacter felis

CIP 104382

Negative

Negative

Helicobacter mustelae

CIP 103759

Negative

Negative

Helicobacter pullorum

CIP 104787

Negative

Negative

Helicobacter pylori (3)

CIP 103995, CIP 26695, CIP 101260

Negative

Negative

Wolinella succinogenes

CCUG 13145

Negative

Negative

Arcobacter butzleri

CCUG 30485

Negative

Negative

Arcobacter cryaerophilus

CIP 104014

Negative

Negative

Listeria monocytogenes (3)

CIP 103575, ATCC 895807, ATCC 19115

Negative

Negative

Listeria innocua (3)

CCUG 15531, ENVN-INRA

Negative

Negative

Salmonella enterica serovar enteridis

ENVN-INRA

Negative

Negative

S. enterica serovar typhimurium

ATCC 13311

Negative

Negative

Enterococcus faecalis (2)

CIP 103013, CCUG 19916

Negative

Negative

Escherichia coli

V517

Negative

Negative

Pseudomonas aeruginosa (2)

ENVN-INRA

Negative

Negative

Enterobacter aerogenes (2)

ENVN-INRA

Negative

Negative

Staphylococcus aureus (2)

ENVN-INRA

Negative

Negative

Yersinia ruckeri

ATCC 29473

Negative

Negative

Y. ruckeri fish isolates (5)

ENVN-INRA

Negative

Negative


n, number of strains

NCTC, National Collection of Type Cultures (Colindale, UK); CCUG, Culture Collection University of Göteborg (Göteborg, Sweden); ATCC, American Type Culture Collection (Manassas, Va); CIP, Collection of the Pasteur Institute (Paris, France); Anses: Strains from the collection of the French Agency for Food Safety (Ploufragan, France); CNR-CH: Strains isolated from the collection of the French National Reference Center for Campylobacter and Helicobacter (Bordeaux, France); ENVN-INRA: Strains isolated from our in-house collection

Leblanc-Maridor et al. BMC Microbiology 2011 11:113   doi:10.1186/1471-2180-11-113

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