H2O2- and HOCl-induced ATP changes in bacterial pathogens. Bacteria were exposed to reagent H2O2 or HOCl, in vitro, to determine the effect of the oxidant on ATP production as measured by the BacTiter-Glo Microbial Cell Viability Assay (Promega). Concentrations of oxidants used were based on the amounts necessary to eradicate CFU viability as assessed in the previous experiments. A) All organisms displayed significant reduction in ATP production (One-way ANOVA) in an H2O2 dose-dependent manner up to 5 mM. B) ATP production by KP was statistically unaffected by HOCl exposure up to 0.1 mM according to one-way ANOVA (p = 0.53) while all other organisms tested displayed significant HOCl dose-dependent reduction in ATP production in this concentration range. Error bars represent standard deviation of at least n = 3 experiments.
Bonvillain et al. BMC Microbiology 2011 11:112 doi:10.1186/1471-2180-11-112