H2O2 and HOCl-induced membrane permeability. Bacteria were exposed to reagent A) H2O2 or B) HOCl as indicated, and the effect of the oxidant on membrane integrity was measured by the BacLight Bacterial Viability and Counting Kit (Molecular Probes). Membrane integrity of PsA, SA, and KP were not significantly affected by H2O2 up to 5 mM by single-factor ANOVA analyses. All organisms tested demonstrated HOCl dose-dependent membrane permeability except SA which remained unaffected up to 0.1 mM. Error bars represent standard deviations of at least n = 3 experiments.
Bonvillain et al. BMC Microbiology 2011 11:112 doi:10.1186/1471-2180-11-112