Influence of NO and NO synthase (A) on the cell concentration and (B) the percentage of spores per cell during the development of biofilms of B. subtilis 3610 and 3610Δnos at the liquid-air interface as determined by plate counting. Biofilms of wild-type 3610 were grown in 25 mL MSgg medium in glass tubes without supplementation (control), supplemented with 100 μM L-NAME (NOS inhibitor), 75 μM c-PTIO (NO scavenger), and 130 μM Noc-18 (NO donor). Error bars indicate standard deviation (N = 3).
Schreiber et al. BMC Microbiology 2011 11:111 doi:10.1186/1471-2180-11-111