Western blot analysis of cadherin protein expression. (A) Percent index variance analysis of the western blot showing cadherin expression: (C1) 2-day old uninfected cultures, (C2) 3-day old uninfected SkMC (control), (I) cultures after 24 h of interaction with T. gondii tachyzoites, and (P) parasites alone (confirming the absence of synthesis of cadherin by T. gondii tachyzoites). Quantitative analysis revealed only 10% reduction in the expression of cadherin between normal cultures, reaching values of more than 50% reduction in T. gondii infected SkMC after 24 h. Results are representative of three independent experiments. Student's T-test (*) p ≤ 0.05.
Gomes et al. BMC Microbiology 2011 11:110 doi:10.1186/1471-2180-11-110