Figure 4.

1.2% agarose gel showing results of semi-nested PCR with primers RFA12/RFA13 and RFA12/P2 specific for Coccidioides spp., lines 1, 5, 9, 13 and 17 = white, lines 2-4 DNA C. posadasii (pure), lines 6-8 DNA C. posadasii (diluted at 10-2), lines 10-12 DNA C. posadasii (diluted a 10-3), lines 14-16 DNA C. posadasii (diluted 10-4). MW = 1 Kb DNA Ladder (Promega).

de MacĂȘdo et al. BMC Microbiology 2011 11:108   doi:10.1186/1471-2180-11-108
Download authors' original image