Table 1

PCR, E-test and FISH results of the detection of clarithromycin resistance in H. pylori clinical isolates

Strain

Genotype

PCR

E-test

PNA-FISH


39391

WT

S

S

S

22771

WT

S

R

S

24061

WT

S

S

S

24241

WT

S

S

S

31831

WT

S

S

S

31311

WT

S

S

S

31481

WT

S

S

S

2452A1

WT

S

S

S

24481

WT

S

S

S

27081

A2142G

R

R

R

27121

A2142G

R

R

R

39411

WT/A2143G

R

R

R

21911

A2143G

R

R

R

20091

A2143G

R

R

R

11621

A2143G

R

R

R

19871

A2143G

R

R

R

20531

A2143G

R

R

R

25381

A2143G

R

R

R

25391

A2143G

R

R

R

27681

A2143G

R

R

R

1662

A2142C

R

R

R

1672

A2143G

R

R

R

1682

A2142G

R

R

R

1692

WT

S

S

S

7.833

A2143G

R

R

R

7.113

A2142G

R

R

R

7.383

A2142G

R

R

R

62713

A2142G

R

R

R

7.363

A2142G

R

R

R

62313

A2142G

R

R

R

9681

WT

S

S

S

NCTC 116374

WT

S

S

S

ATCC 7003924

WT

S

S

S


Some strains were also sequenced in our labs to guarantee that no contamination had occurred during culture maintenance

1 Dr. M. Oleastro (National Institute of Health, Lisbon, Portugal); 2Dr. R. Haas (Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University of Munich, Germany); 3Dr. G. Perez-Perez (NYU Langone Medical Center, New York, USA); 4 Type strain; R: Clarithromycin resistant (MIC > 1 μg/ml); S: Clarithromycin susceptible (MIC < 1 μg/ml); WT: Wild Type.

Cerqueira et al. BMC Microbiology 2011 11:101   doi:10.1186/1471-2180-11-101

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