Specificity of FISH experiments. Hybridization of fixed cells of F. alocis (a and c) and F. villosus (b and d) was performed with probes EUB 338-Cy5 (magenta, a and b) and FIAL-Cy3 (bright orange, c and d). (a and c): Identical microscopic fields show detection of F. alocis by both EUB 338 (a) and FIAL (c) whereas detection of F. villosus by EUB 338 only (b) and not FIAL (d) proves specificity of the FISH experiment.
Schlafer et al. BMC Microbiology 2010 10:66 doi:10.1186/1471-2180-10-66