Table 2

Oligonucleotide probes and hybridisation conditions used in the analysis of faecal bacteria by FISH and FCM

Probe

Target Bacterial group

Sequence (5'-3')

Hybridisation Conditions (°C)

References


Eub 338

Domain bacteria

GCT GCC TCC CGT AGG AGT

50

[13]

Non 338

Negative control

ACA TCC TAC GGG AGG C

50

[14]

Bif 164

Bifidobacterium

CAT CCG GCA TTA CCA CCC

50

[24]

Lab 158

Lactobacillus/Enterococcus

GGT ATT AGC A(C/T)C TGT TTC CA

45

[25]

Bac 303

Bacteroides/Prevotella

CCA ATG TGG GGG ACC TT

45

[26]

Ecol 1513

Escherichia coli

CAC CGT AGT GCC TCG TCA TCA

50

[27]

Chis 150

Clostridium histolyticum

TTA TGC GGT ATT AAT CT(C/T) CCT TT

50

[28]

C Lis 135

Clostridium lituseburense

GTT ATC CGT GTG TAC AGG G

50

[28]

FPrau 645

Faecalibacterium prausnitzii

CCT CTG CAC TAC TCA AGA AAA AC

50

[29]

SRB 687

Sulphate-reducing bacteria

TAC GGA TTT CAC TCC T

50

[30]

STA

Staphylococcus

TCC TCC ATA TCT CTG CGC

50

[31]


De Palma et al. BMC Microbiology 2010 10:63   doi:10.1186/1471-2180-10-63

Open Data