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Open Access Highly Accessed Research article

Intestinal dysbiosis and reduced immunoglobulin-coated bacteria associated with coeliac disease in children

Giada De Palma1, Inmaculada Nadal1, Marcela Medina1, Ester Donat2, Carmen Ribes-Koninckx2, Miguel Calabuig3 and Yolanda Sanz1*

Author Affiliations

1 Ecofisiología Microbiana y Nutrición, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Apartado 73, 46100 Burjassot, Valencia, Spain

2 Hospital Universitario La Fe, Avenida Campanar 21, 40009 Valencia, Spain

3 Hospital General Universitario, Avenida Tres Cruces s/n 46014 Valencia, Spain

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BMC Microbiology 2010, 10:63  doi:10.1186/1471-2180-10-63

Published: 24 February 2010

Abstract

Background

Coeliac disease is a chronic intestinal inflammatory disorder due to an aberrant immune response to dietary gluten proteins in genetically predisposed individuals. Mucosal immune response through IgA secretion constitutes a first line of defence responsible for neutralizing noxious antigens and pathogens. The aim of this study was the characterization of the relationships between immunoglobulin-coated bacteria and bacterial composition of faeces of coeliac disease (CD) patients, untreated and treated with a gluten-free diet (GFD) and healthy controls.

Results

IgA-coated faecal bacterial levels were significantly lower in both untreated and treated CD patients than in healthy controls. IgG and IgM-coated bacterial levels were also significantly lower in treated CD patients than in untreated CD patients and controls. Gram-positive to Gram-negative bacteria ratio was significantly reduced in both CD patients compared to controls. Bifidobacterium, Clostridium histolyticum, C. lituseburense and Faecalibacterium prausnitzii group proportions were less abundant (P < 0.050) in untreated CD patients than in healthy controls. Bacteroides-Prevotella group proportions were more abundant (P < 0.050) in untreated CD patients than in controls. Levels of IgA coating the Bacteroides-Prevotella group were significantly reduced (P < 0.050) in both CD patients in comparison with healthy controls.

Conclusions

In CD patients, reduced IgA-coated bacteria is associated with intestinal dysbiosis, which altogether provide new insights into the possible relationships between the gut microbiota and the host defences in this disorder.