RT-PCR targeting the unique vlhA derived transcript. RT-PCR amplification of DNAse I-treated whole M. synoviae RNA using a sense primer (PromF) located at the 5'-end region of the expected vlhA transcript and a reverse primer (2/28.1Rev) located at the 3' end of MS2/28.1 coding sequence (lane 2). As negative control, PCR was directly performed on RNA without RT (lane 1). DNA size marker (1 kb) (lane M).
Béjaoui Khiari et al. BMC Microbiology 2010 10:6 doi:10.1186/1471-2180-10-6