Table 1

Reactivity of mAbs MEST-1, -2 and -3, with different fungi preparation

MEST-1

Galfβ1→3/6Manp

MEST-2

GlcCer

MEST-3

Manpα1→3Manpα1→2Ins




HPTLC

IFI

HPTLC

IFI

HPTLC

IFI


Pb

Y

+

+

+

+

+

+

M

+

-

+

-

+

-

Ss

Y

- (np)

- (np)

+

+

+

+

M

- (np)

- (np)

+

-

- (np)

- (np)

Hc

Y

+

+

+

+

+

+

M

- (np)

- (np)

+

-

- (np)

- (np)


Reactivity of mAbs MEST-1, -2 and -3, with fungal glycolipids by HPTLC immunostaining (HPTLC); and with fixed fungi by indirect immunofluorescence (IFI). Pb = P. brasiliensis; Ss = S. schenckii; Hc = H. capsulatum; Y = yeast; M = mycelium; MEST-1 recognizes epitope Galfβ1→3/6Manp; MEST-2 recognizes fungal glucosylceramide (GlcCer); MEST-3 recognizes epitope Manpα1→3Manpα1→2Ins; "+" indicates positive staining; "-" indicates negative staining, and "np" indicates epitope not present. [13,24].

Toledo et al. BMC Microbiology 2010 10:47   doi:10.1186/1471-2180-10-47

Open Data