Effect of mAbs on mycelia formation. Yeasts were suspended in 1 ml of PGY or BHI medium. This suspension was added to a 24-well plate and supplemented with mAb MEST-1 or -3 (50 μg/ml), after one hour at 37°C cells were placed at 25°C. After 24 and 48 h of incubation yeast differentiation into mycelia forms was observed in an inverted microscope. Controls experiments were performed identically in presence of irrelevant immunoglobulins (normal mouse total Ig).
Toledo et al. BMC Microbiology 2010 10:47 doi:10.1186/1471-2180-10-47