Inhibition of mAb MEST-3 binding to Pb-2. 96-well plates were adsorbed with GIPC Pb-2 from mycelium forms of P. brasiliensis. Methyl-glycosides, disaccharides and GIPC-derived glycosylinositols (first well 100 mM) were serially double diluted with PBS and preincubated with MEST-3, and the inhibition assay was carried out as described in Materials and Methods. The effects of the methyl-glycosides, disaccharides and glycosylinositols are expressed as percentages of inhibition of MEST-3 binding to Pb-2. (closed square) Manpα1→2Manp, (closed circle) Manpα1→3Manp, (closed triangle) Manpα1→6Man, (open diamond) methyl-α/β-D-glucopyranoside; (open circle) methyl-α/β-D-galactopyranoside; (open triangle) methyl-α/β-D-mannopyranoside, (closed diamond) Manα1→3Manα1→2Ins, (open square) Manα1→3Manα1→6Ins.
Toledo et al. BMC Microbiology 2010 10:47 doi:10.1186/1471-2180-10-47