Table 2

Real-time PCR quantification of bifidobacteria and lactobacilli

Subject

Time point

16S rrn operons/μg fecal genomic DNA (mean ± SD)


Bifidobacterium

B. longum

Lactobacillus

L. helveticus


1

T0

9.4 × 106 ± 3.7 × 106

3.2 × 106 ± 1.5 × 106

2.6 × 106 ± 9.6 × 105

0.0 ± 0.0

T1

4.1 × 106 ± 8.3 × 105

1.1 × 106 ± 2.9 × 105

1.9 × 106 ± 9.9 × 105

4.5 × 102 ± 2.9 × 102

2

T0

8.9 × 107 ± 3.1 × 107

4.2 × 107 ± 3.6 × 107

1.1 × 105 ± 5.6 × 104

9.0 × 101 ± 6.2 × 101

T1

1.6 × 107 ± 5.0 × 106

4.7 × 106 ± 2.9 × 105

5.1 × 105 ± 2.4 × 105

2.6 × 103 ± 2.8 × 102

3

T0

4.0 × 108 ± 3.6 × 107

8.6 × 106 ± 2.6 × 106

5.6 × 104 ± 3.5 × 104

0.0 ± 0.0

T1

2.4 × 108 ± 2.5 × 107

2.4 × 107 ± 2.9 × 106

2.6 × 105 ± 1.6 × 105

2.8 × 103 ± 1.8 × 103

4

T0

2.6 × 108 ± 2.8 × 107

2.3 × 107 ± 2.9 × 106

1.6 × 105 ± 1.0 × 103

2.1 × 103 ± 8.7 × 101

T1

5.8 × 108 ± 1.2 × 107

3.7 × 107 ± 3.1 × 106

1.2 × 105 ± 2.7 × 104

1.6 × 103 ± 2.2 × 102

5

T0

3.1 × 106 ± 8.6 × 105

9.8 × 105 ± 2.8 × 105

1.9 × 104 ± 5.8 × 103

0.0 ± 0.0

T1

2.4 × 106 ± 7.3 × 105

9.5 × 105 ± 3.4 × 105

6.1 × 104 ± 3.4 × 104

3.5 × 102 ± 2.3 × 102

6

T0

1.7 × 108 ± 3.8 × 107

6.5 × 106 ± 2.4 × 105

2.7 × 105 ± 1.2 × 105

0.0 ± 0.0

T1

6.2 × 108 ± 4.2 × 107

3.5 × 107 ± 2.0 × 105

1.7 × 105 ± 1.1 × 105

0.0 ± 0.0

7

T0

6.4 × 107 ± 4.8 × 106

3.4 × 107 ± 1.2 × 106

4.0 × 105 ± 1.7 × 105

9.0 × 101 ± 8.2 × 101

T1

7.5 × 107 ± 1.2 × 106

4.6 × 107 ± 5.5 × 106

9.2 × 105 ± 4.9 × 105

1.4 × 104 ± 3.2 × 103

8

T0

1.8 × 106 ± 5.8 × 105

6.0 × 105 ± 3.6 × 105

1.0 × 106 ± 1.0 × 106

0.0 ± 0.0

T1

4.1 × 106 ± 8.5 × 105

1.3 × 106 ± 9.7 × 105

1.7 × 105 ± 1.7 × 105

0.0 ± 0.0

9

T0

4.4 × 106 ± 2.8 × 105

3.0 × 106 ± 2.3 × 106

9.2 × 105 ± 9.0 × 105

3.0 × 103 ± 1.1 × 103

T1

5.6 × 106 ± 1.4 × 105

3.8 × 106 ± 1.3 × 106

2.0 × 106 ± 1.0 × 106

1.8 × 103 ± 1.7 × 103

10

T0

1.0 × 108 ± 1.8 × 107

7.0 × 107 ± 4.5 × 107

7.7 × 105 ± 7.6 × 105

0.0 ± 0.0

T1

3.3 × 108 ± 7.7 × 107

4.3 × 107 ± 2.5 × 107

1.3 × 106 ± 1.2 × 106

3.2 × 103 ± 2.7 × 103

11

T0

4.1 × 106 ± 7.5 × 105

1.2 × 106 ± 2.5 × 105

5.1 × 105 ± 4.1 × 105

6.0 × 102 ± 3.8 × 102

T1

3.4 × 107 ± 6.2 × 105

3.1 × 107 ± 1.0 × 107

7.8 × 105 ± 7.7 × 105

1.7 × 104 ± 3.1 × 103

12

T0

3.4 × 105 ± 7.6 × 104

7.5 × 102 ± 3.0 × 101

1.7 × 107 ± 1.1 × 107

0.0 ± 0.0

T1

1.3 × 106 ± 7.0 × 105

2.0 × 105 ± 9.3 × 104

5.8 × 105 ± 5.6 × 105

3.6 × 103 ± 6.4 × 102

13

T0

3.5 × 107 ± 1.6 × 106

1.2 × 107 ± 2.6 × 105

1.8 × 105 ± 1.0 × 105

0.0 ± 0.0

T1

2.3 × 107 ± 3.8 × 106

4.6 × 106 ± 4.4 × 105

2.5 × 105 ± 1.8 × 105

1.8 × 102 ± 4.3 × 101

14

T0

1.1 × 107 ± 6.9 × 105

2.3 × 106 ± 1.6 × 106

1.1 × 106 ± 1.8 × 105

0.0 ± 0.0

T1

5.4 × 107 ± 1.7 × 107

1.0 × 107 ± 6.5 × 106

7.2 × 105 ± 6.4 × 105

3.0 × 102 ± 3.0 × 101

15

T0

6.1 × 107 ± 7.4 × 106

1.7 × 107 ± 8.3 × 106

3.9 × 105 ± 2.9 × 105

1.8 × 101 ± 1.6 × 101

T1

2.5 × 107 ± 5.3 × 106

1.0 × 107 ± 5.8 × 106

2.5 × 105 ± 2.2 × 105

3.2 × 102 ± 1.4 × 102

16

T0

1.3 × 109 ± 4.5 × 108

4.0 × 107 ± 1.2 × 107

2.0 × 106 ± 1.1 × 106

0.0 ± 0.0

T1

1.3 × 109 ± 2.0 × 108

2.2 × 107 ± 3.8 × 106

1.0 × 106 ± 8.2 × 105

8.3 × 102 ± 1.4 × 101

17

T0

1.6 × 107 ± 1.6 × 106

5.0 × 106 ± 3.2 × 106

1.3 × 107 ± 2.9 × 106

1.3 × 102 ± 1.1 × 102

T1

2.2 × 107 ± 1.9 × 106

4.0 × 106 ± 2.7 × 106

1.5 × 107 ± 2.0 × 105

6.6 × 102 ± 9.5 × 101

18

T0

1.1 × 105 ± 3.1 × 106

1.4 × 103 ± 4.4 × 102

3.1 × 107 ± 2.7 × 107

0.0 ± 0.0

T1

3.7 × 105 ± 8.9 × 104

1.7 × 105 ± 7.3 × 104

3.0 × 106 ± 1.2 × 106

6.5 × 102 ± 1.2 × 102

19

T0

5.2 × 107 ± 1.7 × 107

4.3 × 105 ± 1.8 × 105

2.5 × 106 ± 1.9 × 106

0.0 ± 0.0

T1

2.0 × 107 ± 8.0 × 106

1.5 × 105 ± 9.4 × 104

2.0 × 106 ± 1.5 × 106

0.0 ± 0.0

20

T0

6.6 × 106 ± 5.2 × 106

4.4 × 106 ± 2.2 × 106

1.0 × 107 ± 8.4 × 106

1.8 × 103 ± 2.6 × 102

T1

7.0 × 106 ± 3.3 × 105

5.5 × 106 ± 3.3 × 106

2.7 × 105 ± 2.6 × 105

0.0 ± 0.0


Vitali et al. BMC Microbiology 2010 10:4   doi:10.1186/1471-2180-10-4

Open Data