Growth of C. efficiens DSM44547 carrying either the empty vector pVWEx1 (squares) or the vector pVWEx1-dld (circles) in CgXII mineral medium containing 100 mM D-lactate and 1 mM IPTG. A representative growth curve is shown. The growth was monitored as OD600nm (closed symbols); the concentration of D-lactate in the supernatant was measured by HPLC (open symbols).
Kato et al. BMC Microbiology 2010 10:321 doi:10.1186/1471-2180-10-321