Table 1

Caco-2 cell genes involved in intracellular junction complex formation that were differentially expressed in the microarray analysis after co-culturing with L. plantarum MB452 (OD600 nm 0.9) for 10 hours.

Gene Name

Symbol

Refseq ID

Fold Change

Moderated

Description of role in relation to tight junctions


occludin

OCLN

NM_002538

1.39

0.004

tight junction bridging protein

vascular endothelial growth factor A

VEGFA

NM_001025366

1.39

0.002

cytokine that indirectly regulates tight junction formation and strengthening

actin beta

ACTB

NM_001101

1.33

0.005

structural constituent of cytoskeleton

cingulin

CGN

NM_020770

1.29

0.024

tight junction plaque protein associated with occludin

par-6 partitioning defective 6 homolog beta

PARD6B

NM_032521

1.27

0.009

tight junction plaque protein associated with claudins and involved in cell polarization

actin alpha cardiac muscle 1

ACTC1

NM_005159

1.25

0.015

structural constituent of cytoskeleton

itchy homolog E3 ubiquitin protein ligase

ITCH

NM_031483

1.25

0.011

ubiquitin-ligase molecule that regulates occludin degradation

junction plakoglobin

JUP

NM_002230

1.24

0.010

major cytoplasmic protein that forms a complex with cadherins

CNKSR family member 3

CNKSR3

NM_173515

1.24

0.006

tight junction plaque protein associated with JAMs

snail homolog 1

SNAI1

NM_005985

1.24

0.033

intracellular component that indirectly inhibits occuldin production

hepatocyte nuclear factor 4 alpha

HNF4A

NM_178849

1.24

0.021

transcription regulator that acts on occuldin

zona occludens 1 (tight junction protein 1)

ZO-1

NM_003257

1.23

0.013

tight junction plaque protein associated with occludin, JAMs and claudins

zona occludens 2 (tight junction protein 2)

ZO-2

NM_004817

1.23

0.054

tight junction plaque protein associated with occludin and claudins that acts as a guanylate kinase and also found in the nucleus

CD2-associated protein

CD2AP

NM_012120

1.22

0.012

scaffolding molecule that regulates the actin cytoskeleton

vinculin

VCL

NM_003373

1.22

0.027

cytoskeletal protein

membrane associated guanylate kinase 3

MAGI-3

NM_152900

1.21

0.044

tight junction plaque protein a and guanylate kinase

membrane protein, palmitoylated 5

MPP5

NM_022474

1.20

0.014

tight junction plaque protein associated with claudins and guanylate kinase involved in tight junction organization

cleavage and polyadenylation specific factor 2

CPSF2

NM_017437

-1.22

0.022

transcription regulator that decreases tight junction stability

cyclin-dependent kinase 4

CDK4

NM_000075

-1.30

0.011

transcription regulator that decreases tight junction stability


Anderson et al. BMC Microbiology 2010 10:316   doi:10.1186/1471-2180-10-316

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