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Open Access Highly Accessed Research article

Lactobacillus plantarum MB452 enhances the function of the intestinal barrier by increasing the expression levels of genes involved in tight junction formation

Rachel C Anderson1*, Adrian L Cookson1, Warren C McNabb23, Zaneta Park4, Mark J McCann1, William J Kelly5 and Nicole C Roy13

Author Affiliations

1 AgriFoods & Health Section, Food & Textiles Group, AgResearch Grasslands, Private Bag 11008, Palmerston North 4442, New Zealand

2 Food & Textiles Group, AgResearch Grasslands, Private Bag 11008, Palmerston North 4442, New Zealand

3 Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand

4 Bioinformatics, Mathematics & Statistics Section, Applied Biotechnology Group, AgResearch Grasslands, Private Bag 11008, Palmerston North 4442, New Zealand

5 Ruminant Nutrition & Microbiology Section, Food & Textiles Group, AgResearch Grasslands, Private Bag 11008, Palmerston North 4442, New Zealand

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BMC Microbiology 2010, 10:316  doi:10.1186/1471-2180-10-316

Published: 9 December 2010

Additional files

Additional file 1:

Summary of microarray analysis. Contains Tables S1, S2 and S3 which summarise the differentially expressed IPA Functional Groups, Gene Ontology categories and KEGG pathways, respectively.

Format: DOC Size: 44KB Download file

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Additional file 2:

Identification of L. plantarum MB 452 from VSL#3. Describes the Pulse-field gel electrophoresis and 16 s sequencing methods used to identify L. plantarum MB 452.

Format: DOC Size: 27KB Download file

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Additional file 3:

Analysis of gene expression of Caco-2 cells treated with L. plantarum MB452. Describes the microarray analysis and qRT-PCR analysis. Include Table S4 showing the qRT-PCR primers.

Format: DOC Size: 74KB Download file

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Open Data