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Open Access Highly Accessed Methodology article

Development and validation of a FACS-based lipoprotein localization screen in the Lyme disease spirochete Borrelia burgdorferi

Ozan S Kumru1, Ryan J Schulze12, Joyce G Slusser1 and Wolfram R Zückert1*

Author Affiliations

1 Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA

2 University of Bristol, Department of Biochemistry, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK

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BMC Microbiology 2010, 10:277  doi:10.1186/1471-2180-10-277

Published: 3 November 2010

Additional files

Additional file 1:

Table S1. Phenotypes of OspA20:mRFP1 fusion mutants

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Additional file 2:

Figures S1 and S2. Protease accessibility and membrane localization of OspA:mRFP1 fusion mutants.

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