Table 6

Detection of BoNT DNA from purified DNA of bacterial cultures or extracted DNA from crude toxin supernatants

BoNT A

BoNT B

BoNT C

BoNT D

BoNT E

BoNT F

BoNT G


BoNT subtype

strain

ABI

LC

ABI

LC

ABI

LC

ABI

LC

ABI

LC

ABI

LC

ABI

LC

A1

Hall

++++

+++

A2b

CDC 1436

++

++++

+++

A3

Loch Maree

++

++++

B1

Okra

++++

+++

B2

213B

++++

++

B2

CDC 1828

++++

+++

B3

CDC 795

+++

+++

B4 (npB)

Eklund 17B

++

+++

Ba4

CDC 657

+

+

+++

+++

Bf

An436

+++

+++

++

+++

C

Stockholm

++++

+++

C/D

6813

++

++

++

D

ATCC 11873

++

+++

D/C

VPI 5995

++

++++

+++

E1

Beluga

++++

++

E2

CDC 5247

++++

++

E2

CDC 5906

+++

++

E3

Alaska E43

++++

+++

E4 (It butyr)

BL5262

+++

++

F1 (prot)

Langeland

++++

+++

F2 (np)

Eklund 202F

+++

++

F3 (baratii)

Orange

++

G

1354

++++

+++

C.sporogenes

-

-

-

-

-

-

-

-

-

-

-

-

-

-

106 standard

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

105 standard

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

++++

104 standard

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

103 standard

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

102 standard

+++

+++

+++

+++

+++

++

+++

+++

+++

+++

+++

+++

+++

++

negative

-

-

-

-

-

-

-

-

-

-

-

-

-

-


ABI = Extracted DNA run on an ABI 7700 real time PCR sequence detector.

LC- = Crude C. botulinum culture supernatants run on the Roche Light Cycler. 0-20 cycles = ++++,

21-30 cycles = +++, 31-40 cycles = ++, > 41 cycles = +

Listed in this table are all strains tested by quantitative PCR for type-specific BoNT. All serotype primer and probe sets were tested against all strains indicated. Standards indicate the plasmid standards used to determine the quantity of BoNT DNA in each sample. Strains tested on the ABI 7700 machine (ABI) included purified DNA from bacterial cultures while samples tested by the Roche Light Cycler (LC) were from crude toxin supernatants.

Hill et al. BMC Microbiology 2010 10:267   doi:10.1186/1471-2180-10-267

Open Data