qPCR detection of type-specific BoNT DNA in food samples spiked with purified C. botulinum DNA. Canned green beans or corned beef was spiked with ten-fold dilutions of purified type-specific BoNT DNA. Samples were processed and DNA extracted from each sample. Results show copy number of each type-specific BoNT dilution in both food types.
Hill et al. BMC Microbiology 2010 10:267 doi:10.1186/1471-2180-10-267