Figure 2.

qPCR validation of plasmid standards. Each standard dilution tested against type-specific primers and probes and cross-checked with primers and probes specific to all remaining types. Five ten-fold serial dilutions tested with each appropriate primer and probe set are depicted in each amplification plot. All reactions amplified with non-type-specific primer and probe sets show no amplification and are represented in bottom right amplification plot.

Hill et al. BMC Microbiology 2010 10:267   doi:10.1186/1471-2180-10-267
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