qPCR validation of plasmid standards. Each standard dilution tested against type-specific primers and probes and cross-checked with primers and probes specific to all remaining types. Five ten-fold serial dilutions tested with each appropriate primer and probe set are depicted in each amplification plot. All reactions amplified with non-type-specific primer and probe sets show no amplification and are represented in bottom right amplification plot.
Hill et al. BMC Microbiology 2010 10:267 doi:10.1186/1471-2180-10-267