Selection and design of universal PCR primers. (A) Diagram of C. botulinum neurotoxin gene (BoNT) organization (adapted from Chen et al. 2007) . (B) Non-toxin non-hemagglutinin gene (NTNH) primers targeting a highly conserved area directly upstream from BoNT. Primer sequences contain degenerate bases to accommodate all strain variation.
Hill et al. BMC Microbiology 2010 10:267 doi:10.1186/1471-2180-10-267