Changes in the T. pyriformis population in co-culture with recombinant LLO-prodicing L. innocua. A. Detection of LLO in the culture supernatant of L. innocua and L. monocytogenes. On the left, secreted proteins are separated in the 10 % SDS-PAGE gel; on the right, Western blot analysis of secreted proteins with LLO-specific antiserum; 1 - wild type L. innocua NCTC11288 strain; 2 - L. monocytogenes NCTC 5105 strain; 3 - LLO-expressing L. innocua NCTC11288 (pHly/PrfA*) strain. Numbers show molecular weight standard positions. B. Trophozoite (left) and cyst (right) concentrations related to LLO production: while columns - L. innocua NCTC11288 strain; black columns - LLO-expressing L. innocua NCTC11288 (pHly/PrfA*) strain. Data represent mean ± SE of two experiments made in triplicate. * p < 0,05; **p < 0,005.
Pushkareva and Ermolaeva BMC Microbiology 2010 10:26 doi:10.1186/1471-2180-10-26