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Proteomic identification of secreted proteins of Propionibacterium acnes

Carsten Holland1, Tim N Mak1, Ursula Zimny-Arndt2, Monika Schmid2, Thomas F Meyer1, Peter R Jungblut2 and Holger Brüggemann1*

Author Affiliations

1 Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany

2 Proteomics Core Facility, Max Planck Institute for Infection Biology, Berlin, Germany

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BMC Microbiology 2010, 10:230  doi:10.1186/1471-2180-10-230

Published: 27 August 2010

Additional files

Additional file 1:

Secreted proteins of different P. acnes strains. Bacteria were grown in BHI medium to an OD (600 nm) of 0.6. Proteins in the culture supernatants were precipitated using 10% TCA and separated on 2D-PAGE gels. (A) Second and (B) third replicate of the experiment shown in Figure 1

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Additional file 2:

MS-based identification of all protein spots originating from exponential phase culture supernatants of five P. acnes strains. This table lists all MS-identified proteins that were separated by 2-DE (see Figure 1).

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Additional file 3:

Alternative consequence of guanine stretch alterations upstream of PPA1880. The homopolymeric guanine stretch could be part of the N-terminus of PPA1880. The different lengths of the G tract would lead to the formation of truncated proteins in strains KPA and 266 due to the appearance of a premature stop codon in the respective reading frame. Only in strain P6 a full protein would be synthesized.

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Additional file 4:

Adherence/agglutination of P. acnes strains grown to stationary phase. 2 ml BHI medium per well was inoculated with the indicated five P. acnes strains (OD600 nm 0.01) and grown to stationary phase (72 h) under anaerobic conditions (37°C, 110 rpm). Strain 266 agglutinated stronger than the other strains. Shown are two independent experiments.

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Additional file 5:

MS-based identification of all protein spots originating from the stationary phase culture supernatant of P. acnes strain 266. This table lists all MS-identified proteins that were separated by 2-DE (see Figure 4).

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