Open Access Highly Accessed Methodology article

Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags

Gary D Wu2*, James D Lewis23, Christian Hoffmann16, Ying-Yu Chen2, Rob Knight57, Kyle Bittinger1, Jennifer Hwang1, Jun Chen34, Ronald Berkowsky2, Lisa Nessel23, Hongzhe Li34 and Frederic D Bushman1

Author Affiliations

1 Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6076 USA

2 Division of Gastroenterology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6076 USA

3 Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6076 USA

4 Department of Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6076 USA

5 Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309-0215 USA

6 Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiania, GO, 74001-970, Brazil

7 Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, CO 80309-0215 USA

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BMC Microbiology 2010, 10:206  doi:10.1186/1471-2180-10-206

Published: 30 July 2010

Additional files

Additional file 1:

Table S1. Samples analyzed in the study of methods for storage and DNA isolation. This table summarizes the samples studied comparing methods for storage and DNA isolation.

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Additional file 2:

Table S2. Samples analyzed in the study of variable region primers. This table summarizes the samples used specifically in the analysis of different variable region primers.

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Additional file 3:

Table S3. Sequences of primers used for amplification. This table contains the sequences of primers used for PCR amplification.

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Additional file 4:

Table S4. Samples analyzed in the study of the cloned DNA mock community. This table summarizes the samples used in the study of the cloned DNA mock community.

Format: XLS Size: 34KB Download file

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