Additional file 2.
Figure S2. Examples of flow cytograms and cell cycle analyses of Prochlorococcus marinus PCC9511 cells grown under HL and sampled at different times of the L/D cycle. A, dot plot of green fluorescence from DNA vs. side scatter, for a culture sample taken during the G1 phase, stained with the DNA dye SYBR Green I, then analyzed by flow cytometry. B, FL1 histogram of the same sample as in Fig. A, showing the DNA frequency distribution of Prochlorococcus cells, from which the proportions of cells in G1, S and G2 phases were calculated using the MultiCycle AV™ software. C, same as graph A, but for a culture sample taken during the S phase. D, same as graph B for the sample used to draw graph C. E, same as graph A, but for a culture sample taken during the G2 phase. F, same as graph B for the sample used to draw graph E.
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Kolowrat et al. BMC Microbiology 2010 10:204 doi:10.1186/1471-2180-10-204