Damage of biofilms of S. mutans wildtype and knock-out mutants for comC, comD and comE by carolacton. Biofilms were grown under anaerobic conditions for 24 h and stained with the LIVE/DEAD BacLight Bacterial Viability staining kit. Green and red fluorescence was determined in triplicate samples, and biofilm damage was calculated as reduction of the fluorescence ratio green/red compared to untreated controls. Standard deviations were calculated from 3 - 5 independent experiments.
Kunze et al. BMC Microbiology 2010 10:199 doi:10.1186/1471-2180-10-199