Effect of carolacton concentration on the membrane damage of S. mutans biofilms. Biofilms were grown for 24 h under anaerobic conditions and stained using the LIVE/DEAD BacLight Bacterial Viability kit. Green and red fluorescence was determined, and biofilm damage was calculated as reduction of the fluorescence ratio green/red compared to untreated controls. Each data point is the average of triplicate samples. Standard deviations are given for data points determined in at least three independent experiments.
Kunze et al. BMC Microbiology 2010 10:199 doi:10.1186/1471-2180-10-199