Table 3

Melting temperature (Tm) of each primer according to the number of mismatches allowed between the primer and the target sequence.

Primer

Number of mismatches allowed

0

1*

2*

3*


ITS1(1) **

58.64

51.75+/-2.88

46.51+/-0.6

41.4+/-NA

ITS1(2) **

58.64

52.02+/-2.58

46.46+/-0.87

39.49+/-2.75

ITS1-F

51.04

42.31+/-1.2

38.91+/-2.62

31.64+/-0.67

ITS2

56.68

48.5+/-1.97

39.3+/-2.74

32.99+/-5.67

ITS5

51.64

41.8+/-1.69

36.6+/-3.93

NA

ITS3

56.68

50.6+/-1.15

44.3+/-3.65

39.93+/-7.25

ITS4

50.9

45.04+/-1.3

35.94+/-3.38

32.73+/-1.83

ITS4-B

59.33

54.49+/-2.39

46.6+/-3.06

37.72+/-7.38


* Mean Tm +/- SD is given for primers with 1 or more mismatches as the Tm depends on the type of mismatch.

** ITS1 is evaluated both with the first subset (1) and the second subset (2).

Bellemain et al. BMC Microbiology 2010 10:189   doi:10.1186/1471-2180-10-189

Open Data