Table 2

Percentage of sequences amplified in silico, allowing one mismatch, from ascomycetes, basidiomycetes and 'non-Dikarya' with different primer combinations and using the three sequence subsets 1-3 (see Material and Methods) as templates.

Data subsets

Primer comb.

Ascomycetes

Basidiomycetes

'non-Dikarya'


Subset 1

ITS1*-ITS2

61.21

86.21

88.57

ITS1-F*-ITS2

90.75

99.14

92.38

ITS5*-ITS2

90.84

99.14

98.10

Subset 2

ITS1*-ITS4

61.91

82.00

84.86

ITS3*-ITS4

98.39

73.91

91.04

ITS5-ITS2*

94.89

72.10

92.63

Subset 3

ITS3-ITS4*

94.71

85.55

98.49

ITS3-ITS4-B*

-

39.31

-


* primer evaluated for mismatches within each pair

Bellemain et al. BMC Microbiology 2010 10:189   doi:10.1186/1471-2180-10-189

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