Effects of TSA and 5-dAZA on IL-8 gene expression. (A) HT-29 cells were treated with the indicated concentration of drugs for the indicated times and total RNA was analyzed by real-time PCR. (B) HT-29 cells were pre-treated with 5-dAZA (50 μM) (black bars) or TSA (100 nM) (striped bars) for 24 hours (control) and then treated with LPS (50 ng/ml) and total RNA was extracted at the indicated time points after LPS administration and subjected to real time PCR analysis. The IL-8 mRNA levels were normalized to G6PD levels and expressed as relative to untreated control cells. Data points represent the average of triplicate determinations ± SD. Similar results were obtained in 3 independent experiments. Statistical analyses were performed compared to respective untreated control cells. *, p < 0.01; **, p < 0.05; absence of asterisks = not significant.
Angrisano et al. BMC Microbiology 2010 10:172 doi:10.1186/1471-2180-10-172