Induction of HSV-2-specific neutralizing antibodies in immunized guinea pigs. Two sets of guinea pigs (n = 8; n = 10) were injected s.c. with 5 × 106 PFU/animal of CJ9-gD or with DMEM and boosted after 3 weeks. Blood was taken 3 weeks after each immunization and 5 weeks after challenge. After heat inactivation, serum from each animal was assayed separately for HSV-2-specific neutralizing antibody titers on Vero cell monolayers. The results represent average titers ± SEM. P-value was assessed by Student's t-test (* p < 0.005).
Brans and Yao BMC Microbiology 2010 10:163 doi:10.1186/1471-2180-10-163