Additional file 5.

Figure S4: Activity assay for ferredoxin-NADP+ oxidoreductase (FNR) in the cell extracts of H. modesticaldum. The reaction turnover is monitored by the oxidation of NADPH or NADH at the decrease of A340 with the procedure reported previously [34] (see ref. [34]). Activity assay of NADH versus NADPH (panel A): 0.25 mM NADH or NADPH was used for assaying activity. The reaction rate with NADPH is > 50 fold faster than with NADH, and effect of ferricyanide in the activity of FNR with NADPH as the substrate (panel B).

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Tang et al. BMC Microbiology 2010 10:150   doi:10.1186/1471-2180-10-150