Figure 1.

AThTP levels as a function of time in BL21 cells transferred to minimal medium. (A) The bacteria were grown overnight in LB medium, transferred to M9 minimal medium and incubated at 37°C at 250 rpm in the absence of a carbon source. At the time indicated, 1 mL aliquots were taken for determination of thiamine derivatives. The arrow in (A) indicates the addition of either 10 mM D-glucose, L-lactate, acetate, L-serine or L-glutamate. The inset shows the decrease of AThTP levels on an expanded time scale. (Means ± SD, n = 3)

Gigliobianco et al. BMC Microbiology 2010 10:148   doi:10.1186/1471-2180-10-148
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