Open Access Highly Accessed Open Badges Research article

Phylogenetic analysis and molecular characteristics of seven variant Chinese field isolates of PRRSV

Chengmin Wang1, Bin Wu12, Said Amer14, Jing Luo1, Hongmei Zhang3, Yunhai Guo1, Guoying Dong1, Baohua Zhao2 and Hongxuan He1*

Author Affiliations

1 National Research Center for Wildlife Born Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

2 College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, 050060 China

3 Department of Life Science, Heze College, Heze, Shandong Province 274015 China

4 Department of Zoology, Faculty of Science, Kafr El-Sheikh 33516 Egypt

For all author emails, please log on.

BMC Microbiology 2010, 10:146  doi:10.1186/1471-2180-10-146

Published: 20 May 2010



Porcine reproductive and respiratory syndrome (PRRS) has now been widely recognized as an economically important disease. The objective of this study was to compare the molecular and biological characteristics of porcine reproductive and respiratory syndrome virus (PRRSV) field isolates in China to those of the modified live virus (MLV) PRRS vaccine and its parent strain (ATCC VR2332).


Five genes (GP2, GP3, GP4, GP5 and NSP2) of seven isolates of PRRSV from China, designated LS-4, HM-1, HQ-5, HQ-6, GC-2, GCH-3 and ST-7/2008, were sequenced and analyzed. Phylogenetic analyses based on the nucleotide sequence of the ORF2-5 and NSP2 showed that the seven Chinese isolates belonged to the same genetic subgroup and were related to the North American PRRSV genotype. Comparative analysis with the relevant sequences of another Chinese isolate (BJ-4) and North American (VR2332 and MLV) viruses revealed that these isolates have 80.8-92.9% homology with VR-2332, and 81.3-98.8% identity with MLV and 80.7-92.9% with BJ-4. All Nsp2 nonstructural protein of these seven isolates exhibited variations (a 29 amino acids deletion) in comparison with other North American PRRSV isolates. Therefore, these isolates were novel strain with unique amino acid composition. However, they all share more than 97% identity with other highly pathogenic Chinese PRRSV strains. Additionally, there are extensive amino acid (aa) mutations in the GP5 protein and the Nsp2 protein when compared with the previous isolates.


These results might be useful to study the genetic diversity of PRRSV in China and to track the infection sources as well as for vaccines development.