Additional file 2.
Analysis of surface exposure of HmuY. Analysis of surface exposure of P. gingivalis HmuY analyzed by whole-cell ELISA. P. gingivalis wild-type (A7436, W83) and hmuY deletion mutant (TO4) strains were grown in basal medium supplemented with hemin (Hm) or dipyridyl (DIP). The cells were washed and diluted with PBS (starting at OD660 = 1.0). Varying dilutions of P. gingivalis cells were adsorbed on the wells of the microtiter plate and reacted with pre-immune serum (A) or purified pre-immune IgGs (pre) (B) and immune anti-HmuY serum (A) or purified immune anti-HmuY IgGs (im) (B). Representative data are shown.
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Olczak et al. BMC Microbiology 2010 10:134 doi:10.1186/1471-2180-10-134