Analysis of HmuY protein in P. gingivalis culture medium. Detection of HmuY protein in whole culture medium (A) or after fractionation of the culture medium by ultracentrifugation (B) of the wild-type A7436 and the hmuY deletion mutant (TO4) strains performed by SDS-PAGE and Coomassie Brilliant Blue G-250 staining. C, culture medium after removal of the cells by centrifugation; F, centrifuged and filtered culture medium; Cr, concentrated culture medium after centrifugation and filtration; V, outer-membrane vesicles; S, soluble proteins present in culture medium after ultracentrifugation.
Olczak et al. BMC Microbiology 2010 10:134 doi:10.1186/1471-2180-10-134