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Open Access Open Badges Research article

Species specificity, surface exposure, protein expression, immunogenicity, and participation in biofilm formation of Porphyromonas gingivalis HmuY

Teresa Olczak*, Halina Wójtowicz, Justyna Ciuraszkiewicz and Mariusz Olczak

Author Affiliations

Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland

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BMC Microbiology 2010, 10:134  doi:10.1186/1471-2180-10-134

Published: 4 May 2010

Additional files

Additional file 1:

Comparison of HmuY homologues. Comparison of homologous HmuY amino-acid sequences identified in human pathogens (A) and bacteria identified in oral tissues (B). Amino-acid sequences lacking signal peptides are shown. Positions with identical amino acids in more than 30% of the sequences are shown in black boxes and partial homology is indicated in grey boxes. Phylogenetic relationship between homologous HmuY amino-acid sequences (C). Bacteria infecting the oral cavity are shown in bold. The phylogenetic tree was determined with the Neighbor-Joining method. Bootstrap values are included. Pgi, Porphyromonas gingivalis; Pen, P. endodontalis; Pue, P. uenonis; Bfr, Bacteroides fragilis; Bfi, B. finegoldii; Bco, B. coprocola; Bst, B. stercoris; Bdo, B. dorei; Bvu, B. vulgatus; Bov, B. ovatus; Bca, B. caccae; Bth, B. thetaiotaomicron; Bcp, B. coprophilus; Bsp, Bacteroides sp.; Coc, Capnocytophaga ochracea; Cgi, C. gingivalis; Csp, C. sputigena; Lbo, Leptospira borgpetersenii; Lin, L. interrogans; Ssp, Sphingobacterium spiritivorum; Pbi, Prevotella bivia; Por, P. oris; Pbe, P. bergensis; Pti, P. timonensis; Pme, P. melaninogenica; Pve, P. veroralis; Psp, Prevotella sp.; Pta, P. tannerae.

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Additional file 2:

Analysis of surface exposure of HmuY. Analysis of surface exposure of P. gingivalis HmuY analyzed by whole-cell ELISA. P. gingivalis wild-type (A7436, W83) and hmuY deletion mutant (TO4) strains were grown in basal medium supplemented with hemin (Hm) or dipyridyl (DIP). The cells were washed and diluted with PBS (starting at OD660 = 1.0). Varying dilutions of P. gingivalis cells were adsorbed on the wells of the microtiter plate and reacted with pre-immune serum (A) or purified pre-immune IgGs (pre) (B) and immune anti-HmuY serum (A) or purified immune anti-HmuY IgGs (im) (B). Representative data are shown.

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Additional file 3:

P. gingivalis growth in broth cultures and biofilms, and biofilm accumulation. P. gingivalis growth was analyzed by measuring the OD at 660 nm, cell viability by plating cells on ABA plates and colony forming unit (CFU) calculation, and biofilm accumulation by microtiter plate assay.

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