SEM images of monospecies (Candida spp. or P. aeruginosa) and dual species (Candida spp. and P. aeruginosa) biofilms. (A). Adhesion of C. albicans for 90 min, (B). Adhesion of C. albicans and P. aeruginosa for 90 min, (C). Adhesion of P. aeruginosa for 90 min. Note that there are few C. albicans blastospores with some degrading cells and few cells of P. aeruginosa in dual species biofilm in compared to monospecies counterparts. (D) Initial colonization of C. glabrata for 24 h (E). Initial colonization of C. glabrata and P. aeruginosa for 24 h, (F). Initial colonization of P. aeruginosa for 24 h. Note that C. glabrata is less in number with altered morphology while thin and scant biofilm was formed in the presence of P. aeruginosa. (G) Maturation of C. tropicalis for 48 h, (H). Maturation of C. tropicalis and P. aeruginosa for 48 h, (I). Maturation of P. aeruginosa for 48 h. Note the reduction in number and altered morphology of C. tropicalis in dual species biofilm.
Bandara et al. BMC Microbiology 2010 10:125 doi:10.1186/1471-2180-10-125