Table 2

Differentially expressed flagellar genes in the HP0256 mutant.

Proposed Class

TIGR orf no.

Putative gene product (gene)

Expression ratio

p-value


Class I

HP0019

chemotaxis protein (cheV)

1.221

0.026

HP0082

methyl-accepting chemotaxis transducer (tlpC)

0.945

0.378

HP0099

methyl-accepting chemotaxis protein (tlpA)

1.401**

0.112

HP0103

methyl-accepting chemotaxis protein (tlpB)

1.403**

0.05

HP0173

flagellar biosynthetic protein (fliR)

1.000

0.997

HP0244

signal-transducing protein, histidine kinase (atoS)

1.221

0.651

HP0246

flagellar basal-body P-ring protein (flgI)

-

-

HP0325

flagellar basal-body L-ring protein (flgH)

1.113

0.050

HP0326

CMP-N-acetylneuraminic acid synthetase (neuA)

0.904

0.219

HP0327

flagellar protein G (flaG)

0.749

0.238

HP0351

basal body M-ring protein (fliF)

0.889

0.508

HP0352

flagellar motor switch protein (fliG)

1.158

0.176

HP0391

purine-binding chemotaxis protein (cheW)

1.668**

0.004

HP0392

histidine kinase (cheA)

1.202

0.113

HP0393

chemotaxis protein (cheV)

1.176

0.194

HP0584

flagellar motor switch protein (fliN)

1.758**

0.042

HP0599

hemolysin secretion protein precursor (hylB)

1.201

0.366

HP0616

chemotaxis protein (cheV)

1.159**

0.162

HP0684

flagellar biosynthesis protein (fliP)

0.510

0.058

HP0685

flagellar biosynthetic protein (fliP)

0.493

0.066

HP0703

response regulator

0.715

0.158

HP0714

RNA polymerase sigma-54 factor (rpoN)

1.104

0.699

HP0770

flagellar biosynthetic protein (flhB)

0.621

0.162

HP0815

flagellar motor rotation protein (motA)

0.917

0.538

HP0816

flagellar motor rotation protein (motB)

0.651

0.231

HP0840

flaA1 protein

1.296

0.184

HP1041

flagellar biosynthesis protein (flhA)

0.988

0.921

HP1067

chemotaxis protein (cheY)

0.958

0.905

HP1092

flagellar basal-body rod protein (flgG)

1.142

0.140

HP1286

conserved hypothetical secreted protein (fliZ)

1.305

0.544

HP1419

flagellar biosynthetic protein (fliQ)

0.636

0.036

HP1420

flagellar export protein ATP synthase (fliI)

0.687

0.012

HP1462

secreted protein involved in flagellar motility

1.306

0.003

HP1575

homolog of FlhB protein (flhB2)

1.445

0.239

HP1585

flagellar basal-body rod protein (flgG)

0.590

0.019


Class II

HP0114

hypothetical protein

1.230

0.357

HP0115

flagellin B (flaB)

1.906

0.032

HP0295

flagellin B homolog (fla)

1.734

0.179

HP0869

hydrogenase expression/formation protein (hypA)

1.307

0.109

HP0870

flagellar hook (flgE)

1.892*

0.067

HP0906

hook length control regulator (fliK)

1.13**

0.230

HP1076

hypothetical protein

2.595

0.001

HP1119

flagellar hook-associated protein 1 (HAP1) (flgK)

1.300

0.224

HP1120

hypothetical protein

1.199

0.390

HP1154

hypothetical protein (operon with murG)

1.514

0.055

HP1155

transferase, peptidoglycan synthesis (murG)

1.955

0.034

HP1233

putative flagellar muramidase (flgJ)

1.400

0.144


Class III

HP0472

outer membrane protein (omp11)

1.649

0.009

HP0601

flagellin A (flaA)

1.487

0.229

HP1051

hypothetical protein

1.098

0.501

HP1052

UDP-3-0-acyl N-acetylglucosamine deacetylase (envA)

1.648

0.054


Intermediate

HP0165

hypothetical protein

1.226

0.515

HP0166

response regulator (ompR)

1.596

0.057

HP0366

spore coat polysaccharide biosynthesis protein C

0.860

0.419

HP0367

hypothetical protein

1.853

0.008

HP0488

hypothetical protein

0.711**

0.031

HP0907

hook assembly protein, flagella (flgD)

1.271

0.214

HP0908

flagellar hook (flgE)

1.175

0.119

HP1028

hypothetical protein

0.852

0.286

HP1029

hypothetical protein

0.799

0.019

HP1030

fliY protein (fliY)

0.860**

0.308

HP1031

flagellar motor switch protein (fliM)

0.835

0.054

HP1032

alternative transcription initiation factor, sigma28 (fliA)

0.923

0.371

HP1033

hypothetical protein

0.896

0.467

HP1034

ATP-binding protein (ylxH)

0.87**

0.352

HP1035

flagellar biosynthesis protein (flhF)

0.921

0.187

HP1122

anti-sigma 28 factor (flgM)

0.867

0.310

HP1440

hypothetical protein

0.627

0.026

HP1557

flagellar basal-body protein (fliE)

0.652

0.091

HP1558

flagellar basal-body rod protein (flgC) (proximal rod protein)

0.899

0.480

HP1559

flagellar basal-body rod protein (flgB) (proximal rod protein)

1.305

0.194

HP0751

(flaG2)

1.203

0.350

HP0752

flagellar cap protein (fliD)

1.003

0.986

HP0753

flagellar chaperone (fliS)

0.981

0.825

HP0754

flagellar chaperone (fliT)

1.09**

0.400


Not assigned

HP0410

flagellar sheath associated protein (hpaA2)

0.664

0.038

HP0492

flagellar sheath associated protein (hpaA3)

0.256

0.000

HP0797

flagellar sheath associated protein (hpaA)

0.801

0.170


Full array datasets are in public databases as described in Methods. Fold-changes and p-values were calculated based on 4 independent biological replicates as described in Methods. Open reading frames and gene annotations were based on the TIGR database [23]. The genes were classified in different flagellar classes, as previously proposed [8]. Confirmatory analysis by qRT-PCR was performed for genes with *. Values for genes with ** were lost during the initial array data analysis and subsequently recovered using 3 independent replicates. For technical reasons, some array spots could not be analyzed in individual arrays.

Douillard et al. BMC Microbiology 2010 10:106   doi:10.1186/1471-2180-10-106

Open Data