Ablation of RSV F by anti-F dsRNA. Anti-F dsRNA, RSV infection, and immunostaining of A549 monolayer were performed as described under Materials and Methods. Right panel shows the nuclear staining of the same cells using DAPI (Blue). Note the substantial reduction of F (Green) with as low as 3 nM anti-F dsRNA, and reduction to background levels by 20 nM dsRNA.
Bitko and Barik BMC Microbiology 2001 1:34 doi:10.1186/1471-2180-1-34