Table 3

Bacterial strains and plasmids used or created in this study.

Strain or plasmid

Genotype or description

Source or reference

Strains

Escherichia coli

XL1-Blue

K12 supE44 hsdR17 recA1 endA1 gyrA46 thi

Laboratory collection

relA1 lac^- F' [proAB⁺lacI^qlacZ Δ M15 Tn10(tet^R)]

Bacillus subtilis

168

trpC2

[36]

BSIP7004

trpC2 speD::spc

[2]

BFS1850

trpC2mtnK::lacZ

This work^a

BFS1851

trpC2mtnU::lacZ

This work^a

BSHP1850

trpC2speD::spc mtnT::lacZ

This work

BSHP7010

trpC2 mtnS::spc

This work

BSHP7035

trpC2pmtnK::Tn10

This work

Plasmids

pUC19

cloning vector, Amp^R

[37]

pIC333

mini-Tn10 delivery vector, Spc^R, Ery^R

[32]

pMutin4mcs

cloning vector, Erm^R

[31]

pDU1850

pMutin4mcs::mtnK

This work

pDU1851

pMutin4mcs::mtnU

This work

pHPP7010

pUC19mtnS::spc^b

This work

a. This strain has been constructed during the frame of the EC project for the functional characterization of the genome of B. subtilis in Europe b.spc is the spectinomycin resistance gene from Staphylococcus aureus

Sekowska et al. BMC Microbiology 2001 1:15   doi:10.1186/1471-2180-1-15